Thp1 sirna
WebMethods: Monocytes (Human monocytic THP-1 cells) were stimulated with IFN-γ and LPS. CCL2 gene expression was determined by real-time RT-PCR. CCL2 protein was … WebFeb 3, 2024 · THP-1-derived macrophages were transfected with siRNA oligos targeting IL1β, TNFα, IL6 and IL12 p40 or control siRNA for 24 h, and then co-cultured with HUVECs …
Thp1 sirna
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WebOct 2, 2024 · (A) THP1 cells stably expressing a SAP25-mCherry fusion protein were transfected with non-targeting control (NTC) or IFIT1 siRNA and stimulated with 100 … WebTransfection of THP-1 cells with plasmids or small interfering RNA (siRNA) is achieved by electroporation using the Lonza Nucleofector technology (Basel, Switzerland). This …
WebNov 2, 2015 · THP1 is a human monocytic cell line derived from an acute monocytic leukemia patient. It is used to test leukemia cell lines in immunocytochemical analysis of … WebSeptember 2nd, 2014. This protocol presents an efficient and reliable method to transfect human THP-1 macrophages with siRNA or plasmid DNA by electroporation with high …
WebWe define that motif siRNA inhibits the induction when the motif is located at the 3′ or 5′-terminus of siRNA. Using THP1-Lucia ISG cells with various DNA stimulants, we reveal … Webovergrowth. For each transfection sample, prepare oligomer-Lipofectamine 2000 complexes as follows: A. Dilute 20 pmol Stealth RNAi or siRNA oligomer in 50 µl Gibco™ Opti-MEM™ …
WebSirna Silencing Efficiency. THP-1 Cells Were Differentiated and Treated with No Sirna, Control Scrambled [PDF] Related documentation. A Computational Approach for Defining …
WebMar 14, 2024 · 昆明医科大RFect siRNA转染试剂成功转染人非小细胞肺癌细胞（A549）. 昆明医科大学研究团队在探讨长链非编码RNA DICER1-AS1在砷抑制细胞增殖中的作用相关 … to your eternity btvaWebCell-Specific Transfection Protocols. NOTE: Invitrogen Lipofectamine Transfection Reagent Protocols have been optimized for efficiency, viability, and reproducibility across a broad … to your eternity book 3to your eternity anime chaptersWebTransfection of THP-1 Cells Use this procedure to transfect plasmid DNA into THP-1 cells in a 24-well format (for other formats, see Scaling Up or Down Transfections, below). All … to your eternity bonWebMar 5, 2009 · To assess the role of the inflammasome components ASC and NALP3 for the activation of caspase-1 and IL-1β release, we transfected PMA-primed THP-1 cells with … to your eternity capitulo 8 mangaWebFeb 20, 2024 · The knockdown of TrxR using siRNA reduced BTK mRNA and protein expression. A combination treatment with [Au(d2pype)2]Cl and ibrutinib had a synergistic … to your eternity chapter 150WebCheck out videos that might be relevant for performing siRNA / RNAi /miRNA transfection Human Cells - THP-1 Lipofectamine using Lipofectamine® RNAiMAX Transfection … to your eternity cda