High-fidelity cas13
Web23 de ago. de 2024 · 然而，Cas13 普遍存在旁切活性，会造成脱靶效应，并且还可能会存在毒副作用，这成为 Cas13 走向临床应用所面临的最大障碍。 因此，如何降低或消除 Cas13 蛋白的旁切活性，开发更特异的高保真 Cas13 蛋白对基于 RNA 编辑的基因治疗策略研发和后续临床转化具有重要意义。 http://www.globalauthorid.com/WebPortal/AuthorView?wd=GAID10946652&rc=736969
High-fidelity cas13
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Web17 de jan. de 2024 · High-fidelity Cas13 variants show similar RNA knockdown activity to wild-type Cas13 but no detectable collateral damage in transgenic mice or adeno-associated-virus-mediated somatic cell targeting. Web3 de mai. de 2024 · Therefore, we first fused dCas13X.1 with high-fidelity ADAR2dd (with E488Q/T375G, referred as ADAR2dd*) ... High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral effects
Web28 de set. de 2024 · CRISPR-Cas13 systems have recently been employed to degrade RNA in yeast, plants, and mammalian cell lines. However, no systematic study of the potential of Cas13 has been carried out in an animal system. Here, we show that CRISPR-RfxCas13d (CasRx) is an effective and precise system to deplete specific mRNA … Web11 de ago. de 2024 · High-fidelity Cas13 variants show similar RNA knockdown activity to wild-type Cas13 but no detectable collateral damage in transgenic mice or adeno …
Web🚨High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral effects. Improving Cas13 with rational design + protein engineering... Web近年来随着研究的不断深入，越来越多的研究表明Cas13蛋白在真核细胞内同样具备collateral cleavage能力，这造成细胞休眠、细胞生长缓慢、细胞死亡等不良结果。. 基于上述背景，神经所杨辉团队开发了高保真（High …
Web18 de fev. de 2024 · A high-fidelity RNA-targeting Cas13 restores paternal Ube3a expression and improves motor functions in Angelman syndrome mice. Author links open overlay panel Jinhui Li 1 6, Zhixin Shen 1 6, Yajing Liu 1 6, Zixiang Yan 3 6, Yuanhua Liu 1 6, Xiang Lin 4, Junjie Tang 1, Ruimin Lv 1, Guannan Geng 1, Zhi-Qi Xiong 1, Changyang …
Web18 de fev. de 2024 · A high-fidelity RNA-targeting Cas13 restores paternal Ube3a expression and improves motor functions in Angelman syndrome mice. Author links open … cynthia bryant californiaWeb11 de ago. de 2024 · High-fidelity Cas13 variants show similar RNA knockdown activity to wild-type Cas13 but no detectable collateral damage in transgenic mice or adeno … cynthia buchananWeb23 de dez. de 2024 · Thus, high-fidelity Cas13 variants with minimal collateral effect are now available for targeted degradation of RNAs in basic research and therapeutic … billy reilich bodybuilderWeb12 de abr. de 2024 · rpa虽然用的也较多，不过rpa需要最后一步将扩增产物转化为rna分子，因此，它经常与cas13酶结合使用,它最主要的优势是rpa反应的温度跟crispr反应温度接近，但是最大局限性是和crispr扩增的产物浓度较低。它在靶标浓度低的时候灵敏度不如lamp好。 cynthia brysonWeb29 de nov. de 2024 · They discovered a family of CRISPR-Cas systems containing cas1, cas2, cas4, and a new gene cas14. cas14 encodes for a small Cas protein (40-70 kDa), which is half the size of other Cas proteins found in the so-called class 2 CRISPR-Cas systems. There are 24 variants of the cas14 gene that cluster into 3 subgroups (cas14a-c). cynthia b. taylorhttp://english.cebsit.cas.cn/News/ResearchNews/2024Year/202408/t20240812_311404.html billy reilly shantyWeb23 de fev. de 2024 · Here, we demonstrated that RNA targeting of paternal Ube3a-ATS with a high-fidelity CRISPR-Cas13 (hfCas13x.1) system could restore Ube3a expression to similar levels as that of maternal Ube3a in the cultured mouse neurons. Furthermore, ... billy reilich twitter