Eha105 agrobacterium strain
WebThe EHA105 strain is useful for transgenic operations of rice, tobacco and other plants. EHA105 contains a rifampicin resistance gene (rif). EHA105 strain also contains an …
Eha105 agrobacterium strain
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WebStrains EHA105 was generated from EHA101 through site-directed deletion of the kanamycin resistance gene from the Ti plasmid, otherwise the strains are identical. This … WebGoldBio’s EHA105 ElectroCompetent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our …
WebMar 6, 2024 · Two Agrobacterium strains, EHA105 and LBA4404, both harboring the p35SGUSINT plasmid, carrying the reporter gene for β-glucuronidase ( gus) and the marker gene neomycin phosphotransferase ( nptII ), were used to test the efficiency of the process. WebDec 1, 2024 · Four of the most frequently used strains, GV3101, EHA101, EHA105, and AGL-1, are C58 derivatives and, as such, C58 is by far the most commonly used …
WebAgrobacterium tumefaciens strain GV3101 exhibits antibiotic properties, partially and negatively affected by the T-DNA in pTiC58, that directly kill Xoo, whereas strains LBA4404 and EHA105 inhibit the Xoo-induced HR and non-host resistance by blocking hydrogen peroxide (H 2O 2) accumulation. RESULTS Agrobacterium tumefaciens GV3101 blocks … WebJan 21, 2024 · We chose Agrobacterium strain EHA105, which is widely used in stable genetic transformation in Populus species [36, 37], for the initial evaluation. This strain contains the reporter binary vector Super : GFP - Flag or CaMV 35S : GUS - intro for easy visualization of transient expression.
WebJun 28, 2016 · An effective transformation protocol for Dunaliella, a β-carotene producer, was developed using the synergistic mechanism of D-glucose and Acetosyringone on three different Agrobacterium strains (EHA105, GV3101 and LBA4404). In the present study, we investigated the pre-induction of Agrobacterium strains harboring pMDC45 binary …
WebOct 14, 2024 · Plasmid and Agrobacterium strains Auxotrophs for methionine were generated for Agrobacterium tumefaciens EHA105 and LBA4404 using a protocol developed for constructing Pseudomonas syringae pv. Tomato DC3000 mutants with minor modifications (Kvitko and Collmer 2011 ). black of women\u0027s tennis crosswordWebOct 14, 2024 · Auxotrophic strains of Agrobacterium tumefaciens can contribute to the development of more efficient transformation systems, especially for crops historically … black of sweatshirtWebGoldBio’s EHA105 Agrobacterium Electrocompetent Cells provide high transformation efficiencies ideal for applications such as cDNA or gDNA library construction. EHA105 strain also contains an amber basic Ti … black of the shoulder topWebDec 1, 2014 · Agrobacterium -mediated genetic transformation is the most preferred strategy utilized for plant genetic transformation. The present study was carried out to … garden hoses expandable 100 ftWebMar 24, 2024 · Gene, strain, binary vector, and Agrobacterium culture. For transformation studies, Agrobacterium tumefaciens strain EHA105 harboring the binary vector pCAMBIA2301 was used. The T-DNA region carries uidA or GUS–A reporter gene and was initiated by CaMV35S promoter and NOS terminator sequences, and the kanamycin … garden hoses and fittingsWebApr 17, 2024 · The Agrobacterium strain, EHA101 (Hood et al. 1986) was tested side-by-side against the strain, EHA105 (Hood et al. 1993). Considerable increase in shoot regeneration and transformation was observed with EHA105 compared to EHA101 (Fig. 2a). While shoot regeneration frequency and transformation frequency of 23.5% and 17.5%, … garden hoses on amazonWebAgrobacterium strains and transformation vector The Agrobacterium tumefaciens strains AGL1, EHA105, GV3101, and MP90 transformed with the binary vector pBI121 … garden hose splitter 2 way ebay