WebRT @TheCejrowski: #Cejrowski o pomnikach i inwigilacji "Powinniśmy inwigilację wobec władzy stosować: czy nie biorą łapówek, czy nie kradną, czy nie dogadują ... WebFunction. Thought to be a Golgi-localized beta-glycan synthase that polymerize the backbones of noncellulosic polysaccharides (hemicelluloses) of plant cell wall. Required for synthesis of a cell wall polysaccharide essential for root hair elongation, but not initiation. May be the functional ortholog of rice CSLD1.
The Wojciech Cejrowski DLA ZUCHWAŁYCH on Twitter: …
WebDec 14, 2024 · Abstract. CSLD3, a gene of the cellulose synthase-like D family, affects root hair elongation, but its interactions with ethylene signaling and phosphate-starvation are poorly understood.Here, we aim to understand the role of CSLD3 in the context of the ethylene signaling and phosphate starvation pathways in Arabidopsis plant growth. CSLD3 full coding sequence was amplified by polymerase chain reaction with reverse transcription (RT–PCR) from RNA isolated from A. thaliana seedlings with primers A and B (primer sequences are listed in Supplementary Table S1). Full-length coding sequences for csld3 mutant alleles (kjk-3, kjk-4) were … See more A. thaliana lines used in this study were derived from the wild-type (Col-0) background. Two previously reported CSLD3 mutant … See more Six-day-old A. thaliana seedlings were fixed in PEM buffer (50 mM PIPES, 5 mM EGTA, 5 mM MgSO4 at pH 6.9) containing 4% … See more Bright-field microscopy was carried out using a Nikon Eclipse E600 wide-field microscope with a ×10 Plan Apo (0.45 NA) lens. Images of growing root hairs were collected every 5 s from seedlings in a perfusion chamber … See more Seven-day-old wild-type (Col-0) seedlings were transferred to a perfusion-chamber system for imaging34, and left to equilibrate for 30 min in 0.25× MS liquid medium. After normal root-hair elongation was observed for 15 … See more highway 710 california
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WebHere, we use a combination of genetic rescue experiments with CSLD-CESA chimeric proteins, in vitro biochemical reconstitution, and supporting computational modeling and … WebWe also find that eYFP-CSLD3 proteins, but not CESA cellulose synthases, localize to a polarized plasma-membrane domain in root hairs. Using biochemical methods and genetic complementation of a csld3 mutant with a chimaeric CSLD3 protein containing a CESA6 catalytic domain, we provide evidence that CSLD3 represents a distinct (1→4)-β-glucan ... WebApr 6, 2024 · In addition, while CESA6 and CSLD3 proteins assembled into similar sized complexes, in planta, these CESA protein complexes further assemble into higher-order cellulose synthase complexes. Whether this … small sports cones