Cisplatin cck8
WebCCK8 assay was performed to assess cell proliferation and chemosensitivity of cisplatin-resistant A549/DDP and H1299/DDP cells. Colony-forming assay was utilized to detect colony numbers. Cell migration and invasion ability were measured by transwell assay. The protein levels of LRP, Pgp, MRP1, and SIX1 were examined by Western blot assay. WebSep 15, 2024 · Cisplatin Injection (cisplatin injection (cisplatin (cisplatin injection) injection) ) is a sterile aqueous solution, available in 50, 100 and 200 mL multiple dose …
Cisplatin cck8
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WebWe established sublines that are resistant to cisplatin from EGFR wild-type cells (A549 and H460) and EGFR mutant cells (PC-9 and HCC827). The EGFR-related signals were examined by Western blotting. MTT assay and the trypan blue exclusion method were used for the in vitro study, while tumor size and the SUV of the 18FDG-PET scans were … WebMar 27, 2024 · Cisplatin-induced cell killing was analyzed by CCK8 assay. Intracellular ROS levels were detected by fluorescence-based flow cytometry analysis. In vitro overexpression and knockdown of GPX1 expression were performed using GPX1 expression vector and siRNA approaches.
WebSep 30, 2024 · Cisplatin was the first platinum-based agent shown to have anti-tumor properties. 2 Cisplatin-based combinations remain first-line therapy for a number of solid tumors including lung, ovarian, and esophageal cancer. 3 It induces DNA damage through DNA cross-linking, thus interfering with the fundamental processes of DNA replication … WebCisplatin is a chemotherapy drug which is used to treat cancers including: sarcoma, small cell lung cancer, germ cell tumors, lymphoma, and ovarian cancer. While it is often …
WebMethods: We use CCK8 assay to show synergistic cytotoxicity of COE and cisplatin. Then, PI single staining and FITC-Annexin V/PI double staining were used to observe apoptotic cells through flow cytometry. The proteins of caspase signaling pathway were examined by Western blotting. Web Construction of cisplatin-resistant Siha cell line. (A) Morphology of Siha-N and Siha-DDP. (Magnifications, 200×). (B) Cell viability under the increasing concentration of cisplatin …
WebCisplatin is a chemotherapy drug that contains the metal platinum. It stops or slows growth of cancer cells and other cells that grow rapidly by damaging their DNA. FDA Approved …
WebExosome-transmitted circVMP1 facilitates the progression and cisplatin resistance of non-small cell lung cancer by targeting miR-524-5p-METTL3/SOX2 axis Hongya Xie , Jie Yao , Yuxuan Wang & Bin Ni Pages 1257-1271 Received 24 Jan 2024, Accepted 14 Mar 2024, Published online: 25 Apr 2024 Download citation how to tag people in gmailWebJul 4, 2024 · To investigate whether miR‐320a regulates laryngeal carcinoma cell cisplatin resistance, miR‐320a overexpression HEp‐2 cells were treated with various concentration of cisplatin. CCK8 assays demonstrated that miR‐320a overexpression significantly inhibited HEp‐2 cell viability (Figure 4 E). how to tag people in outlookWebMar 15, 2024 · In vivo experiment showed that pretreatment with anemoside B4 effectively adjusted body weight and kidney index, and reduced cisplatin-elevated blood urea nitrogen (BUN) and creatinine (CREA) levels, as well as ameliorated the histopathological damage. readworks about homelessness answersWebCisplatin (DDP) has been a significant tumor chemotherapy against various solid malignant tumors, ... Cell counting kit-8 assay. Cell viability was measured using the CCK-8 (SAB Biotech., College Park, MD, USA) assay. Cells were cultured in 96-well plates at 3 × 10 3 cells/well for 12 h. Following treatment for 48 h, the CCK-8 reagent was ... readworks abraham answersWebCCK8 was used to detect cell viability and proliferation. Western blotting was implemented to detect the levels of DNA repair-related proteins. Transwell assay was used to detect cell invasion. Results DNA repair was positively associated with the … how to tag people in fb pageWebA cisplatin-resistant cell subline, HeLa/CDDP cells, showed a 19-fold resistance to cisplatin compared with the parent cells. The subline showed a collateral sensitivity to paclitaxel. An equitoxic dose (IC50) of cisplatin produced DNA fragmentation in HeLa cells but not in HeLa/CDDP cells. Transfection of wild-type p53 gene enhanced the ... readworks a special delivery answer keyWebObjective: To investigate the regulatory effect of miR-29b on gastric cells' resistance to cisplatin. Methods: The expression of miR-29b in gastric cancer cell line treated with cisplatin concentration gradient was detected using quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) and Western blotting. CCK8 was used to … readworks a volcano erupts